Fluorescence nanoscopy. Methods and applications
J Requejo-Isidro - Journal of chemical biology, 2013 - Springer
Journal of chemical biology, 2013•Springer
Fluorescence nanoscopy refers to the experimental techniques and analytical methods used
for fluorescence imaging at a resolution higher than conventional, diffraction-limited,
microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses
on the latest and promising developments in acquisition techniques, labelling strategies to
obtain highly detailed super-resolved images and in the quantitative methods to extract
meaningful information from them.
for fluorescence imaging at a resolution higher than conventional, diffraction-limited,
microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses
on the latest and promising developments in acquisition techniques, labelling strategies to
obtain highly detailed super-resolved images and in the quantitative methods to extract
meaningful information from them.
Abstract
Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.
Springer
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