Functional expression and characterization of plant ABC transporters in Xenopus laevis oocytes for transport engineering purposes
Transport engineering in bioengineering is aimed at efficient export of the final product to
reduce toxicity and feedback inhibition and to increase yield. The ATP-binding cassette
(ABC) transporters with their highly diverse substrate specificity and role in cellular efflux are
potentially suitable in transport engineering approaches, although their size and high
number of introns make them notoriously difficult to clone. Here, we report a novel in planta
“exon engineering” strategy for cloning of full-length coding sequence of ABC transporters …
reduce toxicity and feedback inhibition and to increase yield. The ATP-binding cassette
(ABC) transporters with their highly diverse substrate specificity and role in cellular efflux are
potentially suitable in transport engineering approaches, although their size and high
number of introns make them notoriously difficult to clone. Here, we report a novel in planta
“exon engineering” strategy for cloning of full-length coding sequence of ABC transporters …