The agricultural food production should be increased 70% as the global population will reach 9.1 billion people by 2050. Farmers use chemical spray to reduce the loss and for quick management of pest/disease. The availability and affordability of chemicals to marginal farmers are not possible for pest/disease and weed management. The biopesticide industry is a ray of hope among farmers who are interested in organic farming/natural farming for pest and disease management. The consumers are demanding for low-residue and safer product for consumption. Training is needed for farmers to reduce resistance and develop awareness about biopesticide use in various agroecosystems. In this chapter, we will discuss recent importance and prospect of biopesticide in agriculture and the mode of action of biopesticide against insects and diseases. Moreover, special emphasis is given on case studies of pest and disease management through various biopesticides under laboratory as well as field condition.