… genes to define important regulatory features of the gene under study, either using transient
expression protocols … that contain the isolated gene as a stable genetic unit integrated in the …

… In addition to the transfectron protocols, we will describe three highly sensitive luminometric
reporter-gene assays that we routinely use in our laboratory for the analysis of DNA …

… There is no general protocol for gene transfer into plants. Each cell type, tissue, and … transfer
conditions to reach the highest efficiencies and reproducibility in terms of gene expression. …

… As you attempt to express genes transferred into cells or into an animal, you must deal …
cis-acting DNA elements that, either directly or indirectly, affect gene expression. Each …

… Thanks to this truly interdisciplinary group of international scientists, the present book
contains an original collection of protocols for gene expression as well as some overviews and …

… , gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences,
of important genes (eg , homeotic genes) whose gene … Fourth, gene transfer technology …

… protocols for the preparation of "expression constructs" and their introduction into appropriate
host cells and/or organisms. The chapters in this book are grouped by their expression …

… Moreover, there are no viruses or bacteria known to infect chloroplasts that could be used
as vectors for gene transfer. Therefore, early attempts to transform plastids involved the …

… the propriety of clinical trials involving gene transfer as well as the extent and … gene transfer
into human hepatocytes and HCT in animal models, that were submitted to the Human Gene …

… It will deal specificallywith current and forthcoming clinical protocols for gene transfer into …
of gene therapy for malignant and nonmalignant disease is to transfer and express genes on a …