… extremes of naïve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs),
which … Here we report the detailed protocol to generate and characterize the epiblast-like cells (…

… Checking gene expression revealed that common late BC-lineage cell markers were … by
Pluripotent/Epiblast-like, TE-like, and PE-like genes (Figures 2H–2J). The Epiblast-like region …

… -implantation epiblast-derived stem cells), express transcription … the major somatic cell types
as well as primordial germ cells. The … operate to maintain pluripotency in epiblast-like cells. …

… from Epiblast-like cells (EpiLCs), which are transiently induced from ESCs by Activin A and
bFGF and represent an intermediate state from naıve ESCs to primed Epiblast Stem cells. …

… For example, mESCs cannot be efficiently induced into PGCs unless they were first converted
into a metastable type of epiblast-like cells (EpiLCs), while EpiSCs are almost devoid of …

… Although the efficiency of generating aggregates surrounded by nHyCs was similar for …
) data showed that AQP3 was upregulated in epiblast-like cells by co-culture with nTB (…

… We tested whether epiblast-like cysts could recapitulate events … –ventral patterning of
epiblast-like cysts by supplementing … of the microfluidic device for generating epiblast-like cysts, P-…

… epiblast-like cells as epiblast-like stem cells (EpiLSCs). EpiLSCs display metastable
formative pluripotency with bivalent cellular … We develop single-cell stage label transfer (scSTALT) …

… This immortal cell line passes genomic and mitochondrial DNA to the next generation and
during embryogenesis creates “mortal soma” that help the germ line to fulfill its reproductive …

… cells and somatic cells. Here we report the generation of testicular somatic cell-like cells (TesLCs)
including Sertoli … (SSC)-like cells via epiblast-like cells (EpiLCs) from embryonic stem …