Toxoplasma gondii is an important food-and waterborne opportunistic pathogen that causes
severe disease in immunocompromised patients. T. gondii has an unusual clonal population
structure consisting of three widespread lineages known as I, II, and III. To establish the
genotypes of strains of T. gondii associated with human toxoplasmosis, we have developed
a set of four highly sensitive and polymorphic nested PCR markers. Multiplex nested PCR
analysis was used to genotype parasites in cerebral spinal fluid samples from 8 of 10 human …

The genotypes of Toxoplasma gondii strains isolated from HIV and non-HIV
immunocompromised patients with cerebral and extracerebral toxoplasmosis were
determined and compared to those of strains isolated from non-immunocompromised
patients in order to identify the possible relationships between parasite genotype and
morbidity of toxoplasmosis. One hundred and ten strains of T. gondii were obtained, either
by cell culture (n= 73), brain biopsy (n= 17) or mouse inoculation (n= 20). Ninety strains …