Glycosylation profiling of heterologous proteins
JA Cremata, R Montesino, O Quintero, R García - Pichia Protocols, 1998 - Springer
The methylotrophic yeast Pichia pastoris has been widely exploited for its high-level
expression of heterologous proteins by recombination of gene sequences of interest with the
methanol-inducible alcohol oxidase gene (AOX1) promoter (1–3). Secreted and cytoplasmic
expression of heterologous proteins at levels equivalent to Escherichia coli and significantly
higher than in Saccharomyces cerevisiae has been achieved (4). In addition, P. pastoris
cultures can be easily scaled up to high cell densities, and as a result, yields are also high …
expression of heterologous proteins by recombination of gene sequences of interest with the
methanol-inducible alcohol oxidase gene (AOX1) promoter (1–3). Secreted and cytoplasmic
expression of heterologous proteins at levels equivalent to Escherichia coli and significantly
higher than in Saccharomyces cerevisiae has been achieved (4). In addition, P. pastoris
cultures can be easily scaled up to high cell densities, and as a result, yields are also high …
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