Serbia has a good climate and soil conditions for growing grapes. In Serbia three growing regions are determined, with a 22 rayon and 77 vineyard areas, for the production of grape and production of a wine with the geographic origin. Based on the Census of Agriculture in 2012, it was found that 13% of the total number of farms are engaged in viticulture, but 92% of the production is performed at less than 50 acres area. In the production of grapes, family farms are in the first place then a small number of large wineries. Data for gross margin (GM) calculations were collected through a questionnaire from grape wine production farms in Pomoravlje region in the period 2011-2014. For calculating the basic elements of gross margin, we have used data on yield and price, by-product price, quantity and value of fertilizers, pesticides, and fuel, and costs for contracted services. GM was calculated as the total value of a production line subtracted by the direct costs for the production line in question (cf. purchased inputs). The yield of grapes in the period 2011-2014, was approximately one tonne higher than the average yield of grapes in Serbia according to official statistics. Average yield was determined at 8.2 t/ha, and varied in the range from 5.5 t/ha up to a maximum of 12 t/ha. This presents relatively normal fluctuations in the yield, which indicates the value of the coefficient of variation (Cv) of 16%. Also grape yield had a tendency of slight decrease with an average annual rate of 0.3%. The average price of grapes in the reporting period was at about 390 EUR/t. A positive value indicates that rate of change of prices in the reporting period tends to increase by 0.77% per annum. The variable costs (VC) of the grape production in this period were on the average 1408 EUR/ha. VC show a tendency to decrease by 0.56% per year and was the most stable production parameter (Cv=10.53%). Reduction in variable costs and the relative stability of the characteristics are favourable for the producers of grapes. Gross margin of the grape production in households was observed at an average level of about 1760 EUR/ha. Characteristic gross margin has significantly higher variability in relation to other parameters indicating the value of Cv of almost 31%. Although there are fluctuations in the values of gross margins, this parameter tends to increase at a rate of 1.06% per annum.