Quantitative estimation of seismic response of various structural systems at the collapse limit state is one of the most significant objectives in Performance-Based Earthquake Engineering (PBEE). Assessing the effects of uncertainties, due to variability in ground motion characteristics and random nature of earthquakes, on nonlinear structural response is a pivotal issue regarding collapse safety prediction. Incremental Dynamic Analysis (IDA) and fragility curves are utilized to estimate demand parameters and seismic performance levels of structures. Since producing these curves based on a large number of nonlinear dynamic analyses would be time-consuming, selection of appropriate earthquake ground motion records resulting in reliable responses with sufficient accuracy seems to be quite essential. The aim of this research study is to propose a methodology to assess the seismic behavior of reinforced concrete frames at collapse limit state via accurate estimation of seismic fragility curves for different Engineering Demand Parameters (EDPs) by using a limited number of ground motion records. Research results demonstrate that accurate estimating of structural collapse capacity is feasible through applying the proposed method offering an appropriate suite of limited ground motion records.