The present study aimed to determine the hemodynamic and oxidative stress caused by gamma-irradiation in both sexes of rats. Materials and Methods: Twenty-four albino rats were randomly divided into four groups as the following: Group 1 and 2: Control. The rats (males and females) served as controls, group 3 and 4: The rats (males and females) were whole body gamma irradiated with 0.763 Gy from Cesium-137 radioactive source. Results: Gamma radiation exposure for two weeks in both male and female rats significantly raised malondialdehyde (MDA) levels. Furthermore, serum AST activity was greatly altered by gamma-radiation, while the ALT activity significantly increased by the gamma radiation in female rats only. Irradiated animals in highly significant elevated blood pressure in males, whiles in low significant blood pressure was increased in females. Gamma-irradiation animals for both sexes resulted in significant decrease in serum calcium (Ca+ 2) and level in both male and female rats. Conclusion, our results demonstrated that gamma-irradiated rats could increase tissue injuries markers and lipid peroxidation productions. Interestingly, the elevated blood pressure by gamma-ray exposure may be returned to high free radicals and changes in calcium ions.