Transgenic potato plants expressing human CYP1A1 and human CYP1A1/yeast NADPH–
cytochrome P450 reductase (YR) fused enzyme were generated from microtubers by the
use of an Agrobacterium transformation system. The transgenic plants S1384 expressing
human CYP1A1 and both F1386 and F1515 expressing the fused enzyme were selected by
kanamycin resistance, PCR analysis, chlortoluron (CT) resistance, and Western blot
analysis. The integration and transcription of the corresponding CYP1A1 genes were …

The transgenic potato plants T1977, co-expressing human CYP1A1, CYP2B6, and
CYP2C19; S1965, expressing human CYP1A1; S1972, expressing human CYP2B6; and
S1974, expressing human CYP2C19 were selected by the combination of kanamycin-
resistance, PCR, 7-ethoxycoumarin O-deethylase assay, and Western blot analysis. The 7-
ethoxyresorufin O-deethylase activity of both T1977 and S1965 expressing CYP1A1 were
27 and 53 times higher, respectively, than that of the control. T1977 exhibited remarkable …