[HTML][HTML] Heterogeneity of BCR-ABL rearrangement in patients with chronic myeloid leukemia in Pakistan
N Tabassum, M Saboor, R Ghani… - Pakistan Journal of …, 2014 - ncbi.nlm.nih.gov
N Tabassum, M Saboor, R Ghani, M Moinuddin
Pakistan Journal of Medical Sciences, 2014•ncbi.nlm.nih.govAbstract Background and Objective: Breakpoint cluster region-Abelson (BCR-ABL)
rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is
derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9
and BCR gene on chromosome 22. This chimeric protein has various sizes and therefore
different clinical behaviour. The purpose of this study was to determine the heterogeneity of
BCR-ABL rearrangement in patients with Ph+ CML in Pakistan. Methods: The study was …
rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is
derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9
and BCR gene on chromosome 22. This chimeric protein has various sizes and therefore
different clinical behaviour. The purpose of this study was to determine the heterogeneity of
BCR-ABL rearrangement in patients with Ph+ CML in Pakistan. Methods: The study was …
Abstract
Background and Objective: Breakpoint cluster region-Abelson (BCR-ABL) rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9 and BCR gene on chromosome 22. This chimeric protein has various sizes and therefore different clinical behaviour. The purpose of this study was to determine the heterogeneity of BCR-ABL rearrangement in patients with Ph+ CML in Pakistan.
Methods: The study was conducted at Civil Hospital and Baqai Institute of Hematology (BIH) Karachi. Blood samples from 25 patients with CML were collected. Multiplex reverse transcription polymerase chain reaction (RT-PCR) was performed to identify various BCR-ABL transcripts.
Results: All 25 samples showed BCR-ABL rearrangements. Out of these, 24 (96%) patients expressed p210 BCR-ABL rearrangements ie 60%(n= 15) had b3a2 and 32%(n= 8) had b2a2 rearrangements. Co-expression of b3a2/b2a2 rearrangement and p190 (e1a3) rearrangement was also identified in two patients.
Conclusion: It is apparent that majority of the patients had p210 BCR-ABL rearrangements. Frequency of co-expression and rare fusion transcripts was very low.
ncbi.nlm.nih.gov
以上显示的是最相近的搜索结果。 查看全部搜索结果