Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain
J Campos, M Martinez-Pacheco… - Antonie van Leeuwenhoek, 1995 - Springer
Antonie van Leeuwenhoek, 1995•Springer
Abstract Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its
high ability to both tolerate and reduce hexavalent chromium [Cr (VI)] to less-toxic trivalent
chromium [Cr (III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr (VI), in both aerobic
and anaerobic conditions, to Cr (III) which remained in the supernatant. Cr (VI) reduction
was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had
no effect. Studies with permeabilized cells and cell extracts showed that the Cr (VI) …
high ability to both tolerate and reduce hexavalent chromium [Cr (VI)] to less-toxic trivalent
chromium [Cr (III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr (VI), in both aerobic
and anaerobic conditions, to Cr (III) which remained in the supernatant. Cr (VI) reduction
was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had
no effect. Studies with permeabilized cells and cell extracts showed that the Cr (VI) …
Abstract
Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its high ability to both tolerate and reduce hexavalent chromium [Cr(VI)] to less-toxic trivalent chromium [Cr(III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr(VI), in both aerobic and anaerobic conditions, to Cr(III) which remained in the supernatant. Cr(VI) reduction was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had no effect. Studies with permeabilized cells and cell extracts showed that the Cr(VI) reductase of strain QC1-2 is a soluble NADH-dependent enzyme.
Springer
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