Multi-protein complexes are ubiquitous and play essential roles in many biological
mechanisms. Single molecule imaging techniques such as electron microscopy (EM) and
atomic force microscopy (AFM) are powerful methods for characterizing the structural
properties of multi-protein and multi-protein–DNA complexes. However, a significant
limitation to these techniques is the ability to distinguish different proteins from one another.
Here, we combine high resolution fluorescence microscopy and AFM (FIONA–AFM) to allow …