High throughput sequencing of multiple transcripts
GM Georgiou, AD Ellington, S Hunicke-Smith… - 2017 - repositories.lib.utexas.edu
2017•repositories.lib.utexas.edu
The present disclosure generally relates to sequencing two or more genes expressed in a
single cell in a high-throughput manner. More particularly, the present disclosure relates to a
method for high-throughput sequencing of pairs of transcripts co expressed in single cells
(eg, antibody VH and VL coding sequence) to determine pairs of polypeptide chains that
comprise immune receptors.
single cell in a high-throughput manner. More particularly, the present disclosure relates to a
method for high-throughput sequencing of pairs of transcripts co expressed in single cells
(eg, antibody VH and VL coding sequence) to determine pairs of polypeptide chains that
comprise immune receptors.
Abstract
The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co expressed in single cells (eg, antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.
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