HqiA, a novel quorum-quenching enzyme which expands the AHL lactonase family

M Torres, S Uroz, R Salto, L Fauchery, E Quesada… - Scientific reports, 2017 - nature.com
… highlighted that HqiA showed no sequence homology with the known prototypic AHL
lactonases or acylases, thus expanding the AHL-degrading enzymes with a new family related to …

Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4

W Dong, Y Cai, Z Xu, B Fu, Q Chen, Y Cui, Z Ruan… - Microbial Cell …, 2020 - Springer
… In this study, QQ enzyme AiiK was expressed at the surface layer of strain L. casei MCJΔ1. …
These probiotic capabilities also expanded the application range of LcAiiK, which suggested …

Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1

W Dong, J Zhu, X Guo, D Kong, Q Zhang, Y Zhou… - Scientific reports, 2018 - nature.com
HqiA, a novel AHL-degrading enzyme type has AHL … that AiiK was an AHL lactonase, which
contributes to a novel method of identifying the AHL lactonases instead of using tandem MS. …

Computational prediction of active sites and ligands in different AHL quorum quenching lactonases and acylases

Z Nain, UK Adhikari, F Abdulla, N Hossain… - Journal of …, 2020 - Springer
… QQ enzyme groups (ie, AHL lactonases and AHL acylases) in … of enzymes as well as within
the lactonase enzymes but the … , we also found that most lactonases are cytoplasmic while …

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

R Billot, L Plener, P Jacquet, M Elias… - Journal of Biological …, 2020 - ASBMB
AHL-degrading lactonases from different protein families were reported, including metallo-β-lactamase,
phosphotriesterase-like lactonase (… -negative bacteria and quorum quenching. A, …

A Novel Screening System to Characterize and Engineer Quorum Quenching Lactonases

K Sompiyachoke, J Bravo, R Sikdar… - Biotechnology and …, 2025 - Wiley Online Library
… We show that this system is applicable to lactonases from diverse protein familieslactonase
activity and will facilitate the identification and engineering of quorum quenching enzymes. …

Overexpressed recombinant quorum quenching lactonase reduces the virulence, motility and biofilm formation of multidrug-resistant Pseudomonas aeruginosa …

MM Sakr, KM Aboshanab, WF Elkhatib… - Applied microbiology …, 2018 - Springer
… From the previous results, it is clear that the quorum quenching activity of the recombinant
Ahl-1 enzyme used in this study can significantly reduce the virulence of the three tested MDR …

Extracellular lactonase-mediated quorum quenching by a novel Bacillus velezensis

SV Ayyappan, K Bhaskaran - FEMS Microbiology Letters, 2022 - academic.oup.com
… The AHL recovery after acidification suggests the enzyme to … AHL degrading lactonases
from B. velezensis were … However, the extracellular expression of AHL lactonases as observed …

Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity

M Malešević, N Stanisavljević, K Novović… - Microbial …, 2020 - Elsevier
… agents as well as to expand the whole arsenal of virulence … a search for novel alternative
therapeutic approaches … As most of AHL lactonases, YtnP and Y2-aiiA enzymes belong to …

A mesophilic phosphotriesterase-like lactonase shows high stability and proficiency as quorum quenching enzyme

M Marone, E Porzio, EA Lampitella, G Manco - Chemico-Biological …, 2023 - Elsevier
Lactonase (PLL) family was discovered as part of a joint effort of our and Dan Tawfik laboratories
[[1], [2], [3], [4]] and includes a group of enzymes characterised by main lactonasenovel