Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene
(neo) and a human coagulation factor IX genomic construct designed for expression of the
encoded protein in sheep milk. Two cloned transfectants and a population of neomycin
(G418)–resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six
transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and
neo transgenes, whereas three produced from the uncloned population contained the …

In the 1990s, researchers working at the Roslin Institute in Edinburgh, Scotland, performed
cloning experiments in collaboration with PPL Therapeutics in Roslin, Scotland, on human
coagulation factor IX, a protein. The team of scientists used the methods identified during
the…In the 1990s, researchers working at the Roslin Institute in Edinburgh, Scotland,
performed cloning experiments in collaboration with PPL Therapeutics in Roslin, Scotland,
on human coagulation factor IX, a protein. The team of scientists used the methods identified …