Human lung cancer cells grown on acellular rat lung matrix create perfusable tumor nodules
The Annals of thoracic surgery, 2012•Elsevier
BACKGROUND: Extracellular matrix allows lung cancer to form its shape and grow. Recent
studies on organ reengineering for orthotopic transplantation have provided a new avenue
for isolating purified native matrix to use for growing cells. Whether human lung cancer cells
grown in a decellularized rat lung matrix would create perfusable human lung cancer
nodules was tested. METHODS: Rat lungs were harvested and native cells were removed
using sodium dodecyl sulfate and Triton X-100 in a decellularization chamber to create a …
studies on organ reengineering for orthotopic transplantation have provided a new avenue
for isolating purified native matrix to use for growing cells. Whether human lung cancer cells
grown in a decellularized rat lung matrix would create perfusable human lung cancer
nodules was tested. METHODS: Rat lungs were harvested and native cells were removed
using sodium dodecyl sulfate and Triton X-100 in a decellularization chamber to create a …
BACKGROUND
Extracellular matrix allows lung cancer to form its shape and grow. Recent studies on organ reengineering for orthotopic transplantation have provided a new avenue for isolating purified native matrix to use for growing cells. Whether human lung cancer cells grown in a decellularized rat lung matrix would create perfusable human lung cancer nodules was tested.
METHODS
Rat lungs were harvested and native cells were removed using sodium dodecyl sulfate and Triton X-100 in a decellularization chamber to create a decellularized rat lung matrix. Human A549, H460, or H1299 lung cancer cells were placed into the decellularized rat lung matrix and grown in a customized bioreactor with perfusion of oxygenated media for 7 to 14 days.
RESULTS
Decellularized rat lung matrix showed preservation of matrix architecture devoid of all rat cells. All three human lung cancer cell lines grown in the bioreactor developed tumor nodules with intact vasculature. Moreover, the lung cancer cells developed a pattern of growth similar to the original human lung cancer.
CONCLUSIONS
Overall, this study shows that human lung cancer cells form perfusable tumor nodules in a customized bioreactor on a decellularized rat lung matrix created by a customized decellularization chamber. The lung cancer cells grown in the matrix had features similar to the original human lung cancer. This ex vivo model can be used potentially to gain a deeper understanding of the biologic processes involved in human lung cancer.
Elsevier
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