Human mobility prediction is crucial for epidemic control, urban planning, and traffic forecasting systems. We observe urban traffic flow prediction has a hierarchical structure, in which human mobility prediction should consider not only the spatial and the temporal relationships, but also the high-level mobility trend between individuals and regions. In this paper, we propose a human mobility clustering algorithm based on trend iteration of spectral clustering (TISC) to incorporate the high-level human mobility trend between individuals and regions. We integrate our TISC clustering algorithm with two existing urban traffic flow predictive models: namely, deep spatio-temporal residual network (ST-ResNet) and deep spatio-temporal 3D network (ST-3DNet). By adapting our TISC clustering algorithm, the prediction accuracy of both algorithms has been improved significantly (30.96 for ST-ResNet and 24.66 for ST-3DNet). We also compare the TISC-based predictive framework with 26 state-of-the-art human mobility prediction algorithms. We observe that our TISC algorithm considerably outperforms all 26 methods, reducing the predictive error from 6.93% to 69.55 .