The microbiological quality of air, surface and working personnel in a pharmaceutical industry was investigated. Bacterial isolates recovered from the processing environment were also identified. Phenol co-efficient of commonly used disinfectants such as sodium hypochlorite, savlon (chlorhexidine gluconate 0.3% w/v and cetrimide 3% w/v), ethanol, methanol and isopropyl alcohol were determined using standard methods against the most predominant organisms. This study reveals that the personnel involved in manufacturing were the main source of contamination and the common organisms found from samples of air, surface, and working personnel monitoring area were Staphylococcus sp., Pseudomonas sp. and Escherichia sp. Among the used disinfectants of this study, sodium hypochlorite showed the highest potency. Phenol coefficient of sodium hypochlorite, savlon, isopropyl alcohol, methanol and ethanol were 5.55 and 4.44;