Identification of “Cronobacter” spp. (Enterobacter sakazakii)
C Iversen, A Lehner, N Mullane, J Marugg… - Journal of Clinical …, 2007 - Am Soc Microbiol
Journal of Clinical Microbiology, 2007•Am Soc Microbiol
ABSTRACT A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to
consist of five species within a new genus,“Cronobacter,” has recently been proposed. The
correct identification of these organisms is important to clinicians. Therefore, using 312
Enterobacteriaceae, including 210 “Cronobacter” strains, the reliabilities of biochemical and
genetic confirmation tests were investigated. All “Cronobacter” isolates were positive using
dnaG and gluA PCR protocols, and all expressed α-glucosidase activity. ID32E v3. 0 …
consist of five species within a new genus,“Cronobacter,” has recently been proposed. The
correct identification of these organisms is important to clinicians. Therefore, using 312
Enterobacteriaceae, including 210 “Cronobacter” strains, the reliabilities of biochemical and
genetic confirmation tests were investigated. All “Cronobacter” isolates were positive using
dnaG and gluA PCR protocols, and all expressed α-glucosidase activity. ID32E v3. 0 …
Abstract
A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist of five species within a new genus, “Cronobacter,” has recently been proposed. The correct identification of these organisms is important to clinicians. Therefore, using 312 Enterobacteriaceae, including 210 “Cronobacter” strains, the reliabilities of biochemical and genetic confirmation tests were investigated. All “Cronobacter” isolates were positive using dnaG and gluA PCR protocols, and all expressed α-glucosidase activity. ID32E v3.0 identified 99.5% of “Cronobacter” isolates (as the nearest match to E. sakazakii).
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