Objective: To assess the incidence and gender distribution of Cystic duct variations visualized during laparoscopic cholecystectomy. Study design: Observational study. Place and Duration: This study was carried out at the department of Surgery, and department of Anatomy Dow University of Health Sciences (DUHS), from Sep 2011 to Sep 2013. Methodology: There were 300 diagnosed cases of Cholelithiasis, undergoing Laparoscopic Cholecystectomy (LC) in Civil Hospital Karachi (CHK) and other hospitals of Karachi. Laparoscopic Cholecystectomy was performed under general anesthesia using the four port technique. The information of Anatomy on laparoscopic visual field was recorded by DVD recorder after obtaining informed consent from the patient. Over all frequencies of cystic duct variations were assessed. The gender distribution of these variations was also evaluated. Results: Out of 300 cases, there were 78.7% female (236) patients and 21.3%(64) male patients. There were 85.7%(257) cases having normal anatomy of the cystic duct and 14.3%(43) cases had anatomical variations. Out of these we found 6.3% Short cystic duct, 3.7% Long Cystic duct, 2.7% Spiral cystic duct, 1.0% Accessory cystic duct, 0.3% Adherent cystic duct and 0.3% absent cystic duct. We found most of these variations in both the gender groups of our population. Conclusion: We found a significant number of cystic duct variations in our patient population which may lead to surgical complication if not handled appropriately. Anatomical details and orientation are required to deal with these variations whenever they come across. we have identified the presence of these variations in both the gender groups.