[PDF][PDF] Identification of four major Meloidogyne ssp. by random amplified polymorphic DNA (RAPD-PCR)
JL Cenis - … -NEW YORK AND BALTIMORE THEN ST PAUL-, 1993 - apsnet.org
PHYTOPATHOLOGY-NEW YORK AND BALTIMORE THEN ST PAUL-, 1993•apsnet.org
The random amplified polymorphic DNA (RAPD) assay is a variant of the polymerase chain
reaction (PCR) in which a single primer of random sequence is used at a low-annealing
temperature. Twenty-two primers were evaluated for their usefulness in identifying 18
populations of Meloidogyne incognita, M. arenaria, M. javanica, and M. hapla collected
worldwide. Reactions with primer OPA-01 produced amplified DNA
reaction (PCR) in which a single primer of random sequence is used at a low-annealing
temperature. Twenty-two primers were evaluated for their usefulness in identifying 18
populations of Meloidogyne incognita, M. arenaria, M. javanica, and M. hapla collected
worldwide. Reactions with primer OPA-01 produced amplified DNA
The random amplified polymorphic DNA (RAPD) assay is a variant of the polymerase chain reaction (PCR) in which a single primer of random sequence is used at a low-annealing temperature. Twenty-two primers were evaluated for their usefulness in identifying 18 populations of Meloidogyne incognita, M. arenaria, M. javanica, and M. hapla collected worldwide. Reactions with primer OPA-01 produced amplified DNA
The American Phytopathological Society