Identification of root-preferential transcription factors in rice by analyzing GUS expression patterns of T-DNA tagging lines
T-DNA tagging lines are useful for analyzing the functions of genes and regulatory elements.
We have previously generated approximately 100,000 insertional mutants in japonica rice
(Oryza sativa), using T-DNA vectors carrying the promoter-less GUS reporter gene. In this
study, we conducted GUS assays of seedlings from 430 lines in which TDNA was inserted
into transcription factor genes. Among the 75 lines that showed GUS signals, nine displayed
an endospermpreferential expression pattern; two lines demonstrated GUS signals in both …
We have previously generated approximately 100,000 insertional mutants in japonica rice
(Oryza sativa), using T-DNA vectors carrying the promoter-less GUS reporter gene. In this
study, we conducted GUS assays of seedlings from 430 lines in which TDNA was inserted
into transcription factor genes. Among the 75 lines that showed GUS signals, nine displayed
an endospermpreferential expression pattern; two lines demonstrated GUS signals in both …
Identification of root-preferential transcription factors in rice by analyzing GUS expression patterns of T-DNA tagging lines.
WJH Wei JinHuan, WYF Wu YunFei… - 2017 - cabidigitallibrary.org
T-DNA tagging lines are useful for analyzing the functions of genes and regulatory elements.
We have previously generated approximately 100,000 insertional mutants in japonica rice
(Oryza sativa), using T-DNA vectors carrying the promoter-less GUS reporter gene. In this
study, we conducted GUS assays of seedlings from 430 lines in which TDNA was inserted
into transcription factor genes. Among the 75 lines that showed GUS signals, nine displayed
an endosperm-preferential expression pattern; two lines demonstrated GUS signals in both …
We have previously generated approximately 100,000 insertional mutants in japonica rice
(Oryza sativa), using T-DNA vectors carrying the promoter-less GUS reporter gene. In this
study, we conducted GUS assays of seedlings from 430 lines in which TDNA was inserted
into transcription factor genes. Among the 75 lines that showed GUS signals, nine displayed
an endosperm-preferential expression pattern; two lines demonstrated GUS signals in both …
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