The present study aimed to investigate the efficiency of alpha hemolysin (Hla) and heat inactivate bacteria suspension (HIBS) antigens prepared from Methicillin Resistant Staphylococcus aureus (MRSA) bacteria isolated from burn wound in rats after immunization. Both antigens examined after the period of immunization to determine the total IgG concentration, IFN-γ and TNF-α levels in serum. The results indicated that Hla antigen had the ability to modulate and induce the humoral and cellular immune response since that there was a significant elevation in serum total IgG and IFN-γ at (p≤ 0.05) in comparison with the HIBS antigen. Resent data also indicated to a significant decreasing in TNF-α concentration in sera of rats immunized with two studied antigens Hla (44.54) pg/ml and HIBS (16.5) pg/ml.