Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy
JG Sykes, J Kuiper, JB Richardson… - Osteoarthritis and …, 2018 - oarsijournal.com
Purpose: High hopes have been pinned on regenerative medicine strategies to meet the
challenge of preventing the progression to osteoarthritis, in particular autologous
chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro
monolayer expansion, a step needed to obtain sufficient cell numbers, may be a key
limitation in ACI. In this study, we determined whether a shorter and quicker monolayer
expansion approach could improve chondrogenic differentiation. Methods: We compared …
challenge of preventing the progression to osteoarthritis, in particular autologous
chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro
monolayer expansion, a step needed to obtain sufficient cell numbers, may be a key
limitation in ACI. In this study, we determined whether a shorter and quicker monolayer
expansion approach could improve chondrogenic differentiation. Methods: We compared …