In vitro determination of the uterine stimulatory effect of the aqueous leaf extract of Ficus exasperata
Journal of Ethnopharmacology, 2010•Elsevier
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Ficus exasperata Vahl
(Moraceae) are used by traditional healers in Southern Nigeria to arrest pre-term
contractions and are also used as an abortifacient in some parts of Africa. AIM OF STUDY:
An earlier study on the aqueous leaf extract of Ficus exasperata (AET) showed that the
extract at lower concentrations inhibited oxytocin-induced uterine contractions and at higher
concentrations, stimulated uterine contraction. This study thus aims to determine, the …
(Moraceae) are used by traditional healers in Southern Nigeria to arrest pre-term
contractions and are also used as an abortifacient in some parts of Africa. AIM OF STUDY:
An earlier study on the aqueous leaf extract of Ficus exasperata (AET) showed that the
extract at lower concentrations inhibited oxytocin-induced uterine contractions and at higher
concentrations, stimulated uterine contraction. This study thus aims to determine, the …
ETHNOPHARMACOLOGICAL RELEVANCE
The leaves of Ficus exasperata Vahl (Moraceae) are used by traditional healers in Southern Nigeria to arrest pre-term contractions and are also used as an abortifacient in some parts of Africa.
AIM OF STUDY
An earlier study on the aqueous leaf extract of Ficus exasperata (AET) showed that the extract at lower concentrations inhibited oxytocin-induced uterine contractions and at higher concentrations, stimulated uterine contraction. This study thus aims to determine, the possible mechanisms by which AET stimulates uterine contraction in vitro.
MATERIALS AND METHODS
The contractile effect of AET (5.0×10−2 to 100×10−2mg/ml) and oxytocin (which was used as a reference drug) were examined in the presence of the following antagonists: atropine (1.18 and 11.91nM); indomethacin (1.42 and 14.25nM); verapamil (2.03 and 20.35nM); phentolamine (4.09 and 40.91nM), or diphenhydramine (4.45 and 44.47nM). The EC50 and Emax were determined and statistically analyzed using one-way ANOVA and Dunnett post hoc test.
RESULTS
There was no significant difference in the EC50 and Emax of AET and oxytocin in the presence of atropine. Diphenhydramine and phentolamine significantly inhibited (p<0.01) the extract but both drugs had no effect on oxytocin. However, significant differences (p<0.01) were observed in the EC50 and Emax of AET and oxytocin in the presence of verapamil and indomethacin.
CONCLUSIONS
These results suggest that the stimulation of uterine contractility by AET may arise from the activation of histamine H1- and/or α-adrenergic receptors, interference with calcium channels and/or stimulation of prostaglandin synthesis in utero.
Elsevier
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