… The physiological functions of endogenous retrovirus, which … -inactivated pigs will shed light
on the endogenous retrovirus … the PERV-inactivated pig can serve as a foundation pig strain, …

… the fetal fibroblast FFF3 cells did not contain PERV- C, and also the surrogate sows used to
… Inactivation of porcine endogenous retrovirus in pigs using CRISPR- Cas9. Science. 2017;…

… of PERV inactivation for XTx, we tested the susceptibility of a PERV RT-inactivated porcine
… PERV and investigated the influence of CRISPR/Cas9-mediated indels on virus particle …

… PERVs raised the question of whether conventional pigs can still be used for xenotransplantation,
or whether only CRISPR/Cas9 inactivated pigs must be used as source animals for …

… level will be required when selecting pigs with low copy number and low expression of
PERV as well as when inactivating PERVs using the clustered regularly interspaced short …

… Using CRISPR-Cas9, we disrupted all copies of the PERV pol gene and demonstrated a >…
cells, using our engineered cells. Our study shows that CRISPR-Cas9 multiplexability can be …

… Pig to human xenotransplantation is considered to be a possible … Porcine endogenous
retrovirus (PERV) is the most significant pathogen in xenotransplantation. We screened for pigs …

… endogenous retroviruses (PERVs) could infect human cells. In the past, we determined
the … the porcine kidney epithelial cell line PK15 and in primary fibroblasts. Using CRISPR-Cas9, …

… Furthermore, we applied CRISPR/Cas9 technology to inactivate and eliminate the
potential risk of cross-species transmission and obtained PERV-destructed PRF clonal cells. …

… Pigs with porcine endogenous retrovirus (PERV) inactivation are preferable donor sources
… as an infectious pathogen for humans who receive pig organ xenotransplantation. However, …