Recurrent pregnancy loss (RPL), which is generally known as >2 consecutive pregnancy losses before 20 weeks' gestation, is seen in between 3-5% of women. The aim is to evaluate the frequency of common variants with hereditary thrombophilia; (Methylenetetrahydrofolate Reductase; MTHFR C677T and MTHFR A1298C polymorphisms; FV Leiden G1691A and Prothrombin G20210A mutation) in female patients with RPL. This retrospective cohort study is between the years 2020 and 2022. We investigated the frequency of the MTHFR C677T and MTHFR A1298C polymorphisms, FV Leiden G1691A and Prothrombin G20210A mutations in 380 females suffering from two or more pregnancy losses. The mean age of the patients was 32.6 years (range: 18-46). The most frequently observed variant in this group was a heterozygote mutation for the MTHFR677 polymorphism (n=163, 42.8%). The second most common variant was MTHFR A1298C heterozygosity with a frequency of 41.1% (n=156). The least common variant is FII homozygosity (0.3%). Heterozygous of FII and FV Leiden (G1691A) mutations were found in 1.8% (1/380) and 8.4% (7/380) of the patients, respectively. The thrombophilic variants (FV Leiden G1691A and Prothrombin G20210A) seem to have an important role in RPL. Detection of an underlying thrombophilia may be important to begin early and appropriate treatment for couples suffering from RPL.