[HTML][HTML] Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
K Dissanayake, M Nomm, F Lättekivi, Y Ressaissi… - Theriogenology, 2020 - Elsevier
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have
gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs
from media conditioned by individually cultured preimplantation bovine embryos and to
assess their relationship with embryo quality. Presumptive zygotes were cultured
individually in 60 μl droplets of culture media, and 50 μl of media were collected from the
droplets either on day 2, 5 or 8 post-fertilization. After sampling, the embryo cultures were …
gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs
from media conditioned by individually cultured preimplantation bovine embryos and to
assess their relationship with embryo quality. Presumptive zygotes were cultured
individually in 60 μl droplets of culture media, and 50 μl of media were collected from the
droplets either on day 2, 5 or 8 post-fertilization. After sampling, the embryo cultures were …
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