influencing the expression of native genes for foundational studies, cellular reprograming,
and metabolic engineering. Here we develop a method for near leak-free, inducible
expression of a polycistronic array containing up to 24 gRNAs from two orthogonal
CRISPR/Cas systems to increase CRISPRai multiplexing capacity and target gene flexibility.
To achieve strong inducibility, we create a technology to silence gRNA expression within the …