Inducible expression of large gRNA arrays for multiplexed CRISPRai applications

WM Shaw, L Studená, K Roy, P Hapeta… - Nature …, 2022 - nature.com
CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for
influencing the expression of native genes for foundational studies, cellular reprograming,
and metabolic engineering. Here we develop a method for near leak-free, inducible
expression of a polycistronic array containing up to 24 gRNAs from two orthogonal
CRISPR/Cas systems to increase CRISPRai multiplexing capacity and target gene flexibility.
To achieve strong inducibility, we create a technology to silence gRNA expression within the …

[引用][C] Inducible expression of large gRNA arrays for multiplexed CRISPRai applications. Nat Commun 13: 4984

WM Shaw, L Studena, K Roy, P Hapeta, NS McCarty… - 2022
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