Sutanto, A. and MA Aziz. 2006. The induction and regeneration of somatic embryogenesis on papaya. The experiments with the objectives to induce and regenerate somatic embryogenesis were carried out at the Tissue Culture Laboratory of Crop Science Department, Universiti Putra Malaysia from January to August 2001. The experiments involved the induction of somatic embryogenesis from immature zygotic embryos of papaya cv. Eksotika II and regeneration of plantlets from the somatic embryos. A nonfactorial completely randomized design was used as experimental design with treatments of the 2.4-D combinations concentrations (1.0; 2.5 and 5.0 mg/l) and BAP concentrations (0; 0.001; 0.005 and 0.01 mg/l). The results showed that MS medium supplemented with 2, 4-D 5.0 mg/l promoted the highest percentage of somatic embryogenesis (74.55%), while the combination of 1.0 mg/l 2, 4-D and 0.01 mg/l BAP produced the highest percentage of callus formation (52.58%) after 8 weeks of culture. The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/l 2, 4-D and 0.01 mg/l BAP were added into MS medium. Maturation of somatic embryos was achieved on transfering the globular somatic embryos derived from zigotic embryo to solid MS medium without growth regulator. After 4 weeks of culture, 42% germinated somatic embryo was occurred following maturation of somatic embryos. Plantlets were ready for acclimatization to germination medium 8 weeks after culture. Somatic embryogenesis system could enhance the successful of the modern papaya breeding program through genetic transformation.