Bacteria have been applied in cement-based materials as a self-healing agent while research about the effect of bacteria on undamaged concrete itself is still limited. This paper investigated influence of bacterial self-healing agent on rheology, hydration process, pore structure, strength development and mineralization product changes. By analyzing zeta potential, electrical resistivity, BET and μ-CT, TG-DSC and SEM results, it could be found that direct incorporation of bacterial self-healing agents has increased consistency, hydration rate, and quantity of mineralization products, which could be conducive to enhance strength and dense pore structure.