This study aimed to determine the influence of ration supplementation with brown seaweed (Turbinaria murayana) on performance, carcass quality, physiological organs, liver fat and broiler liver cholesterol. This study used 100 DOC (day old chicken) broilers of Arbor Acres CP 707 strains. This study used a completely randomized design (CRD) with five treatments; each treatment was repeated four times. The treatments were presented as brown seaweed (Turbinaria murayana), by a rate of 0, 2.5%, 5%, 7.5%, and 10% in broiler ration. Data were analyzed using Analysis of Variance (ANOVA), and Duncan’s Multiple Range Test to reveal the significant differences between different treatments applied. The results showed that the use of Turbinaria murayana up to the level of 10% in the ration can maintain performance, carcass quality, do not interfere with physiological organs, and can reduce the content of abdominal fat, liver fat and broiler liver cholesterol. The study concluded that the use of Turbinaria murayana as a rations supplemental material up to the 10% level can stimulate broilers’ performance and carcass characteristics.