Keratinases are proteases that have several important applications in the daily life. Although
they have attracted the attention of researchers in the field of biotechnology, their use in
industrial processes requires efficient purification techniques allowing scaling-up. Among
these techniques, the aqueous two-phase system and ultrafiltration stand out. This study
reports the purification of keratinase from Bacillus sp. P45 by aqueous two-phase system
two-stage process integrated to ultrafiltration. In the aqueous two-phase system, the addition …