Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection
F Debode, I Huber, R Macarthur, PE Rischitor… - Food Control, 2017 - Elsevier
This paper presents the inter-laboratory studies for the successful validation of qualitative
real-time Polymerase Chain Reaction methods for the detection of Genetically Modified
Organisms in food and feed. Two singleplex TaqMan methods were developed for the
detection of the tE9 terminator present in several genetically modified events and the
detection of the endogenous lectin gene from pea; the tE9 terminator donor organism. In
addition, a TaqMan duplex method was validated, which allows the simultaneous detection …
real-time Polymerase Chain Reaction methods for the detection of Genetically Modified
Organisms in food and feed. Two singleplex TaqMan methods were developed for the
detection of the tE9 terminator present in several genetically modified events and the
detection of the endogenous lectin gene from pea; the tE9 terminator donor organism. In
addition, a TaqMan duplex method was validated, which allows the simultaneous detection …
[引用][C] Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one multiplex (pat/bar) real-time PCR methods for GMO detection
F DEBODE, I HUBER, R MACARTHUR… - publications.jrc.ec.europa.eu
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