Introduction of high throughput and cost effective SNP genotyping platforms in soybean
We presented here the application of two in-plate SNP (single nucleotide polymorphism)
genotyping platforms for soybean plants [Glycine max (L.) Merr.], KASP®(Kompetitive Allele
Specific PCR genotyping, LGC Genomics) and TaqMan®(Life Technologies) respectively.
These two systems offer us an ability to determine the genotypes of 384 individual samples
accurately and efficiently by allele specific PCR in a single plate using typical PCR
conditions. Both of the systems require small quantity of genomic DNA obtained from a …
genotyping platforms for soybean plants [Glycine max (L.) Merr.], KASP®(Kompetitive Allele
Specific PCR genotyping, LGC Genomics) and TaqMan®(Life Technologies) respectively.
These two systems offer us an ability to determine the genotypes of 384 individual samples
accurately and efficiently by allele specific PCR in a single plate using typical PCR
conditions. Both of the systems require small quantity of genomic DNA obtained from a …
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