The isolation, identification and characterization of bacteria from sawdust was carried out. Sawdust waste samples were collected from different spots in the Ahiake saw mill, Umuahia. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic media for cellulolytic count. The pour plate method was employed. Colonial morphology, gram staining and biochemical tests were used for the identification of the bacteria. The total aerobic plate for the sawdust waste ranged from 2.33 x 107 to 8.2 x 106, coliform count ranged from 7.6 x 106to 1.7 x 106Cfu/g and cellulolytic count ranged from 4.7 x 106 to 2.6 x 106Cfu/g. The bacteria isolated from the sawdust waste sample were Pseudomonas spp., Bacillus spp., Escherichia coli, Staphylococcus spp., klebsiella spp., Micrococcus spp., and Cellulomonas spp. The study concludes that microorganisms occur in a large number and variety in sawdust wastes.