A new N-glycosidase ribosome-inactivating protein (RIP) belonging to the novel family of the
nontoxic type 2 RIPs from Sambucaceae has been isolated from rhizomes of dwarf elder
(Sambucus ebulus L.) and named ebulin r. Dwarf elder rhizomes also contain a novel
monomeric N-Ac-galactosamine-binding lectin that we named SEAII. Ebulin r and SEAII
have two isoforms each one, which were readily resolved by ion exchange. Both isoforms of
ebulin (ebulins r1 and r2) strongly inhibited protein synthesis in mammalian but not in plant …

Sambucus (Caprifoliaceae) species contain nigrin b and ebulin I, which are two-chain
ribosomeinactivating proteins (RIPs) belonging to a new type of RIPS which are non-toxic to
mice and cultured human cells. In this work the presence in fruits of elder (S. nigra L.) of a
new non-toxic type 2 RIP (nigrin f) that co-exists with a lectin known as SNA IV is described.
Nigrin f strongly inhibited protein synthesis in mammalian, but not in plant, ribosomes,
promoting the depurination of sensitive ribosomes and thus allowing the release of the RIP …