Diabetes mellitus (DM) is a serious public health problem worldwide (Ozer et al., 2010). Among the 191 WHO members states, India has the highest number of people with diabetes (Chellan et al., 2010). Worldwide, the prevalence of DM has been estimated to be 2.8% in 2000, and projected to be 4.4% in the year of 2030. Foot ulcers are a serious complication of diabetes with recent studies suggesting that life time risk of developing a foot ulcer in diabetic patients may be as high as
25%(Hayat et al., 2011). They are now the most common proximate and nontraumatic causes of leg amputation (Hayat et al., 2011). Various microorganisms colonize the wound and in some patients one or more species of organisms proliferate in the wound, which may lead to tissue damage, host response accompanied by inflammation, that is, clinical infection (Lipsky et al., 2012). Mostly the diabetic foot infections are mixed bacterial infection (Zubair et al.,