Isolation of a new surfactin producer Bacillus pumilus A-1, and cloning and nucleotide sequence of the regulator gene, psf-1
M Morikawa, M Ito, T Imanaka - Journal of fermentation and bioengineering, 1992 - Elsevier
Two bacteria (A-1 and B-1) which exhibited large emulsified halos around their colonies on
oil-L-agar plates were isolated. These bacteria produced the same biosurfactant, surfactin.
Strain A-1 (Psf+: surfactin producing) was identified as Bacillus pumilus and could be
transformed with plasmid DNA using an electroporation method. Several surfactin non-
producing (Psf−) mutants were obtained by chemical mutagenesis of B. pumilus A-1. By
using them as host cells and pC194 as a vector plasmid, we cloned DNA fragments which …
oil-L-agar plates were isolated. These bacteria produced the same biosurfactant, surfactin.
Strain A-1 (Psf+: surfactin producing) was identified as Bacillus pumilus and could be
transformed with plasmid DNA using an electroporation method. Several surfactin non-
producing (Psf−) mutants were obtained by chemical mutagenesis of B. pumilus A-1. By
using them as host cells and pC194 as a vector plasmid, we cloned DNA fragments which …
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