… method to estimate genome-wide DNA methylation, referred to as LUminometric Methylation
Assay (LUMA… Here, we report a novel assay for genomic DNA methylation based on DNA …

… a method to analyze global genomic DNA methylation, using a luminometric technology to
quantitate methylation … for DNA input and can be scaled up for high throughput application. …

… approach 0.0 in the case of full DNA methylation. In this chapter, we describe the Luminometric
Methylation Assay (LUMA), … LUMA results for a sample of human genomic DNA analyzed …

… Luminometric Methylation Assay, or LUMA, in which the radioactive single nucleotide extension
assay … Typical LUMA results for a sample of human genomic DNA analyzed by a PSQ96 …

… In order to test this hypothesis experimentally, we ran several genomic DNA samples on …
global methylation levels by LUMA. As predicted, the fragmented samples had much higher …

… a genomic DNA mark resulting from a covalent bond of a methyl group to the 5-carbon
position of cytosine, generally in a 5′-CpG-3′ context. This dinucleotide is rare in the genome (…

… methylation assays are used to approximate total genomic … throughout the genome and do
not represent all genomic CpG … methylation percentages estimated from the LUMA assay are …

… (Alu, LINE1 and LUMA) for global DNA methylation estimation to the assay of a range of …
inexpensive, and high throughput surrogate assay for overall genomic DNA methylation. In …

… ) is a tool to measure absolute levels of DNA methylation in a given genome. … methylation
sensitive, it should cleave similarly amongst all samples. Additionally, LUMA is high throughput…

… the methyl acceptance assay measures methylation in CpG sequences across the genome,
while LUMA only measures methylation in the … high performance liquid chromatography …