Lactoferrin suppresses LPS-induced expression of HMGB1, microRNA 155, 146, and TLR4/MyD88/NF-кB pathway in RAW264. 7 cells
M Nemati, S Akseh, M Amiri… - …, 2021 - Taylor & Francis
Immunopharmacology and Immunotoxicology, 2021•Taylor & Francis
Objective This current study evaluated the underlying mechanisms of LF against the
inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-кB pathway
in LPS-activated murine RAW264. 7 cells. Methods MTT assay was used to assess cell
metabolism and the cell culture levels of the cytokines (TNF-α, IL-6) were evaluated by
Enzyme-linked immunosorbent assay (ELISA). The expression of miRNAs was quantified by
using qPCR and the expression of HMGB1, TLR4, MyD88, and phosphorylated NF-κB (P …
inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-кB pathway
in LPS-activated murine RAW264. 7 cells. Methods MTT assay was used to assess cell
metabolism and the cell culture levels of the cytokines (TNF-α, IL-6) were evaluated by
Enzyme-linked immunosorbent assay (ELISA). The expression of miRNAs was quantified by
using qPCR and the expression of HMGB1, TLR4, MyD88, and phosphorylated NF-κB (P …
Objective
This current study evaluated the underlying mechanisms of LF against the inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-кB pathway in LPS-activated murine RAW264.7 cells.
Methods
MTT assay was used to assess cell metabolism and the cell culture levels of the cytokines (TNF-α, IL-6) were evaluated by Enzyme-linked immunosorbent assay (ELISA). The expression of miRNAs was quantified by using qPCR and the expression of HMGB1, TLR4, MyD88, and phosphorylated NF-κB (P-p65) were determined with Western blot and qPCR, respectively.
Results
The results indicated that LF downregulates IL-6 and TNF-α expression. LF exhibited the degradation of P-p65 and reduced the production of HMGB1, TLR4, and MyD88 in LPS-induced inflammatory response. Importantly, in parallel with the suppression of cytokines and HMGB1-TLR4-MyD88-NF-кB pathway, LF could induce a decrease in inflammatory selected miRNAs, mmu-mir-155, and mmu-mir-146a expression.
Conclusions
Altogether, these findings provide LF as a prominent anti-inflammatory agent that could modulate HMGB1, mmu-mir-155, mmu-mir-146a, and TLR4/MyD88/NF-кB pathway.
Taylor & Francis Online以上显示的是最相近的搜索结果。 查看全部搜索结果