The influence of turbulence on the minimum ignition energy (MIE) and ignited flame characteristics is investigated for pulsed methane diffusion jets ignited by laser-induced plasma. The methane jet is injected in a volume of homogeneous and isotropic air turbulence without mean flow, with the level of turbulence being controlled independently. The study is carried out for a range of fuel jet (Re_jet) Reynolds number, namely 1000, 2000, and 3000, and a range of turbulent (Re_λ) Reynolds number, namely 0–207. The results show that the position of the maximum intensity of flame emission was randomly scattered due to the fact that the ignited flame is deflected from the nozzle axis by the turbulent velocity fluctuations. The effect is more profound at higher Re_jet. The value of the MIE, determined according to 50% ignitibility of mixture, increases by a factor of 2 for an increase of Re_λ from 0 to 207 and by a factor of 5 for an increase of Re_jet from 1000 to 3000. Two trends are observed on MIE with Re_λ. For low Re_λ, MIE is independent of Re_λ. Past a critical value of Re_λ, MIE increases as a linear function of Re_λ. This transition occurs at critical values of Re_λ,c = 158, 197 and 202 for Re_jet= 1000, 2000 and 3000, respectively. The mean value of MIE for ignition before and after transition is a linear function of Re_jet. The difference between the mean value of MIE before transition and after transition is around 5 mJ for all considered Re_jet.