Core samples from the A‐IG‐85 well and four outcrops located in South Araçoiaba da Serra, São Paulo State, Brazil, have been palynologically investigated. Diverse and well‐preserved palynomorphs, including seventy‐three species of spores, monosaccate, disaccate and taeniate pollen grains, and one species of incertae sedis have been recognized. Among them, sixty species are systematically described including thirty‐four species of spores, twenty‐five pollen grains and one incertae sedis. Eleven species from the Brazilian Paraná Basin are published for the first time: Granulatisporites triconvexus Staplin 1960, Dictyotriletes muricatus (Kosanke) Smith & Butterworth 1967, Vallatisporites punctatus (Marques‐Toigo) comb, nov., Cristatisporites rollerii Ottone 1989, Bascaudaspora canipa Owens 1983, Spelaeotriletes triangulas Neves & Owens 1966, Psomospora detecta Playford & Helby 1968, Florinites occultus Habib 1966, Potonieisporites barrelis Tiwari 1965, Costatascyclus crenatus Felix & Burbridge emend. Urban 1971, Limitisporites luandensis Bose & Maheshwari 1968 including a new combination proposed herein [Vallatisporitespunctatus (Marques‐Toigo) comb. nov.]. The palynofossiliferous beds come from the Ahrensisporites cristatus Interval Zone, in the basal portion of the Itararé Subgroup, and are considered to be late Carboniferous (Westphalian) in age. Changes in the composition of the assemblages and the previous record of marine fossils in the studied beds are interpreted as resulting from minor variations of a transgres‐sive/regressive cycle, under glacial climatic conditions.