Soil pollution has increased in recent years, with the accumulation of heavy metals being a factor necessitating it. In this study, the levels of some metals in the soil collected from four different sites S 1 (400-500m), S 2 (300-250m), S 3 (150-75m) and S 4 (10-25m) around Odo-Efo River that receives effluent from Global soap and detergents industry located at Asa-Dam, Ilorin, Kwara State in north central Nigeria and their effects on biomarker enzymes (ALP and ACP) in earthworm collected from each sites were investigated, as they were compared to a control (S c) soil sample from a forested area at the University of Ilorin botanical garden. The soil samples were assayed for Cd, Cu, Hg and Pb by flame atomic absorption spectrophotometer after aqua ragia digestion. Results of analysis revealed that metal concentration ranged as: Cd (0.003-0.004), Cu (0.04-0.21), Hg (0.001-0.003) and Pb (0.007-0.008) with Cd, Cu and Hg found to be significantly higher (p< 0.05) in all the sampling sites when compared to the control but with a significant decrease (p< 0.05) in the concentration of Pb when compared to the control. The increase in metal concentrations reflected a corresponding increase in the concentration of the earthworm biomarker enzymes (ALP and ACP) and malondialdehyde concentration.