… aptamer ligands, their contributions to background noise, and the impact of tandem aptamer
… Our results suggest the need for caution in using light-up aptamers as reporters for imaging …

… the light-up aptamer progress and applications such as RNA trafficking, metabolite
sensing via a transducer linked to metabolite-sensing aptamers… and visualized by FRET (fluorescence …

… fluorescent reporter for … two aptamers closer to each other and leading to a high FRET
efficiency between Spinach-DFHBI and Mango-YO3-biotin complexes ( Figure 7(b) ). This FRET …

… transfer (FRET, [35]) or contact-mediated quenching [32,55]. However, the presence of an …
allowed establishing the proof-of-concept experiment using light-up aptamers as reporter [84]. …

… reporters on the RNA level. Since intrinsically fluorescent RNAs are not known, the fluorescent
reporter … ) were fused together in a system called “apta-FRET” (Figure 10 C). The fusion of …

… BLAS have been used as reporters of nucleic acid … RNA sequences of the light-up MG
aptamer 10 were linked to … a Förster resonance energy transfer (FRET). Introduced by Stojanovic …

… Light-up RNA aptamers can also be used as reporters to aid in the development of catalytic
… Spinach and Mango aptamers positioned in such a way that FRET can occur between the …

… and another constant fluorescence reporter (fluorescent proteins or light-up RNA aptamers)
that can … Therefore, the presence of analytes could lead to differences in the FRET ratio. By …

Fluorescence turn-on aptamers, in vitro evolved RNA molecules … Turn-on aptamers have been
selected to bind diverse … Nonetheless, each turn-on aptamer has developed idiosyncratic …

… In the past decade, multiple light-up RNA aptamer systems have … two fluorogenic RNA
aptamer reporters: MG aptamer (RNA 1… (Bi-ii) Rigid aptamers are used to measure their FRET …