Longrangedistance NMR effects in a protein labeled with a lanthanideDOTA chelate

MD Vlasie, C Comuzzi… - … A European Journal, 2007 - Wiley Online Library
DOTA-based (DOTA=1,4,7,10-tetraazacyclododecane-N,N′,N′′,N′′′-tetraacetic acid)
lanthanide chelate that is attached to a protein … in the Yb 3+ chelate and the observed PCS …

Site-specific tagging of proteins with paramagnetic ions for determination of protein structures in solution and in cells

XC Su, JL Chen - Accounts of chemical research, 2019 - ACS Publications
… averaging of paramagnetic effects observed in NMR spectra, … for isotope-labeled samples
used for NMR. Cysteine is one of … lanthanide ions complexed with linear and cyclic chelating

Paramagnetic NMR effects of lanthanide ions as structural reporters of supramolecular complexes

CFGC Geraldes - NMR in Supramolecular Chemistry, 1999 - Springer
… of the protein on the basis of the lanthanide effects was not … Ln(DOTA) chelates have also
been extensively studied by NMR.… by the marked increase of relaxivity of GdL chelates bearing …

DOTA-amide lanthanide tag for reliable generation of pseudocontact shifts in protein NMR spectra

B Graham, CT Loh, JD Swarbrick, P Ung… - Bioconjugate …, 2011 - ACS Publications
lanthanide ions to proteins produces large effects in NMR … samples labeled with, respectively,
a paramagnetic or a diamagnetic … Lanthanide chelates with two arms for attachment to two …

Dynamic Exchange of the Metal Chelating Moiety: A Key Factor in Determining the Rigidity of Protein–Tag Conjugates in Paramagnetic NMR

JL Chen, B Li, XY Li, XC Su - The Journal of Physical Chemistry …, 2020 - ACS Publications
… Site-specific labeling of proteins with a paramagnetic tag has … an open-chain chelating
moiety; ie, the cyclic DOTA-like tags … the protein tag:lanthanide molar ratio is ∼1:1, indicating the …

Prospects for lanthanides in structural biology by NMR

G Otting - Journal of biomolecular NMR, 2008 - Springer
long-range effects of paramagnetism, lanthanide labelling will allow the study of larger
proteins and protein… (nitroxide radicals or Mn 2+ chelates) to report on different conformational …

EPR Relaxation‐Enhancement‐Based Distance Measurements on Orthogonally Spin‐Labeled T4‐Lysozyme

S Razzaghi, EK Brooks, E Bordignon… - …, 2013 - Wiley Online Library
… we discuss another lanthanide-based approach for distance … paramagnetic NMR-based
long-range distance measurements … ability of lanthanide ions to substitute each other in chelate

New lanthanide chelating tags for PCS NMR spectroscopy with reduction stable, rigid linkers for fast and irreversible conjugation to proteins

T Müntener, J Kottelat, A Huber… - Bioconjugate …, 2018 - ACS Publications
… a new pyridine thiazole derivate of DOTA, that combines high … In particular paramagnetic
effects like pseudocontact shifts (… a selectively 15 N-Leu labeled construct, which allowed for …

Gd (III) and Mn (II) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins

M Kaushik, T Bahrenberg, TV Can… - Physical Chemistry …, 2016 - pubs.rsc.org
… use of lanthanide chelate tags in NMR and the large availability of respective … mutant
labeled with Gd-DOTA-M; we have observed very similar effects with other point mutation/chelate

DOTA-M8: an extremely rigid, high-affinity lanthanide chelating tag for PCS NMR spectroscopy

D Häussinger, J Huang… - Journal of the American …, 2009 - ACS Publications
… A new lanthanide chelating tag (M8) for paramagnetic labelinglanthanide chelating tag
(LCT) for protein applications. Its … We reasoned that the small paramagnetic effects were related …