Long-depth imaging of specific gene expressions in whole-mount mouse embryos with single-photon excitation confocal fluorescence microscopy and FISH
C Palmes-Saloma, C Saloma - Journal of Structural Biology, 2000 - Elsevier
Long-depth imaging of specific gene expression in the midgestation whole-mount mouse
embryo (WME) is demonstrated with single-photon excitation (1PE) confocal fluorescence
microscopy and fluorescence in situ hybridization. Expression domains of Pax-6 mRNA
transcripts were labeled with an in situ hybridization probe that is a RNA sequence
complementary to the cloned gene fragment and were rendered visible using two
fluorochrome-conjugated antibodies that fluoresce at peak wavelengths of λF= 0.525 μm …
embryo (WME) is demonstrated with single-photon excitation (1PE) confocal fluorescence
microscopy and fluorescence in situ hybridization. Expression domains of Pax-6 mRNA
transcripts were labeled with an in situ hybridization probe that is a RNA sequence
complementary to the cloned gene fragment and were rendered visible using two
fluorochrome-conjugated antibodies that fluoresce at peak wavelengths of λF= 0.525 μm …
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