Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity
S Hofbauer, K Gysel, M Bellei, A Hagmüller… - Biochemistry, 2014 - ACS Publications
Chlorite dismutases (Clds) are heme b containing oxidoreductases that convert chlorite to
chloride and molecular oxygen. In order to elucidate the role of conserved heme cavity
residues in the catalysis of this reaction comprehensive mutational and biochemical
analyses of Cld from “Candidatus Nitrospira defluvii”(NdCld) were performed. Particularly,
point mutations of the cavity-forming residues R173, K141, W145, W146, and E210 were
performed. The effect of manipulation in 12 single and double mutants was probed by UV …
chloride and molecular oxygen. In order to elucidate the role of conserved heme cavity
residues in the catalysis of this reaction comprehensive mutational and biochemical
analyses of Cld from “Candidatus Nitrospira defluvii”(NdCld) were performed. Particularly,
point mutations of the cavity-forming residues R173, K141, W145, W146, and E210 were
performed. The effect of manipulation in 12 single and double mutants was probed by UV …
[引用][C] Manipulating Conserved Heme Cavity Residues of Chlorite Dismutase: Effect on Structure, Redox Chemistry, and Reactivity
H Stefan, G Kira, B Marzia, H Andreas, S Irene… - 2014
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